Cell Line Information:

Designations: 4T1Depositors: BA PulaskiGrowth Properties: adherentOrganism: Mus musculus deposited as mouseMorphology: epithelialOrgan: mammary glandStrain: BALB/cfC3HDisease: tumorTumorigenic: Yes4T1 is a 6-thioguanine resistant cell line selected from the 410.4 tumor without mutagen treatment.When injected into BALB/c mice, 4T1 spontaneously produces highly metastatic tumors that can metastasize to the lung, liver, lymph nodes and brain while the primary tumor is growing in situ.The tumor growth and metastatic spread of 4T1 cells in BALB/c mice very closely mimic human breast cancer. This tumor is an animal model for stage IV human breast cancer. 4T1-induced tumors can also be used as a post-operative model as well as a non-surgical model because the 4T1-induced tumor metastasizes spontaneously in both models with similar kinetics. Because 4T1 is resistant to 6-thioquanine, micro-metastatic cells (as few as 1) can be detected in many distant site organs with better accuracy than most tumor models.

The Transfection Reagent:

4T1 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting 4T1 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for 4T1 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

References:

Garcia-Areas R, Libreros S, Simoes M, Castro-Silva C, Gazaniga N, et al. Suppression of tumor-derived Semaphorin7A and genetic ablation of host-derived Semaphorin7A impairs tumor progression in a murine model of advanced breast carcinoma. Int J Oncol. 2017 Nov;51(5):1395-1404. PubMed PMID: 29048670; PubMed Central PMCID: PMC5642386.

Features:

• Specifically optimized to deliver nucleic acids into 4T1 Cells
• Highest efficiency to ensure experimental success
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• 0.5 ml is able to transfect about 1000 wells of 24-well plate
• Deliver single or multiple plasmids
• Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
• Compatible with serum
• Suitable for Reverse Transfection
• Compatible with transfection in any plate formats
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems