Cell Line Information:

Designations: bEnd.3Depositors: EC ButcherGrowth Properties: adherentOrganism: Mus musculusMorphology: endothelialSource:Organ: brainStrain: BALB/cTissue: cerebral cortexDisease: endotheliomaCell Type: endothelialpolyoma middle T antigen transformedAge: 6 weeksComments: The cells were transformed by infection with the NTKmT retrovirus vector that expresses polyomavirus middle T antigen. The endothelial nature of these cells was confirmed by the observed expression of von Willebrand factor and uptake of fluorescently labeled low density lipoprotein (LDL). The expression of Peyer's Patch high endothelial receptor for lymphocytes, the mucosal vascular addressin (MAdCAM-1) and E-selectin can be induced on bEnd.3 cells by cytokines and lipopolysaccharide (LPS). Intracellular adhesion molecule 1 (ICAM-1) is constitutively expressed on the cells, and expression is increased by treatment with LPS, IL-1 and TNF alpha. Vascular cell adhesion molecule 1 (VCAM-1) is constitutively expressed on the cells at early passages but not at passages over 30.

The Transfection Reagent:

bEnd.3 Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on bEnd.3 Cells. The proprietary formulation of lipids and polymersensures the highest possible transfection efficiencies and viabilities for bEnd.3 Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of bEnd.3 Cell Avalanche™ Transfection Reagent for transfection on bEnd.3 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

References:

Sonar SA, Shaikh S, Joshi N, Atre AN, Lal G.IFN-γ promotes transendothelial migration of CD4⁺T cells across the blood-brain barrier.Immunol Cell Biol. 2017 Oct;95(9):843-853. PubMed PMID: 28682305.

Features:

• Unique formulation-maximize transfection performance in bEnd.3 Cells.
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• 0.5 ml is able to transfect about 1000 wells of 24-well plate
• Deliver single or multiple plasmids
• Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
• Compatible with transfection in any plate formats
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems