Cell Information:

Human Astrocytes are the most abundant cells in the central nervous system. They perform many functions such as provision of mechanical support and nutrients to neurons, and removal of wastes from neurons. In addition to playing a critical support role for optimal neuronal functioning, they also provide biochemical support of endothelial cells which form the blood-brain barrier. Recent studies have shown that astrocytes are capable of regulating neurogenesis by instructing the stem cells to adopt a neuronal fate. They also respond to neurotransmitters and modulate neuronal excitability and synaptic function.

Human Astrocytes are usually derived from human cerebral cortex. They can serve as useful in vitro model for exploring the diversity of astrocytes functions.

Applications:

• Astrocyte-mediated neurotoxicity
• Neurogenesis research
• Injury
• Drug development
• Parkinson's disease
• Alzheimer's disease

The Transfection Reagent:

Transfection of primary cells, such as Human Astrocytes, has always been challenging, both technically and financially. The most common systems currently used for molecular delivery to these cells include expensive electroporation instruments and relatively toxic chemical formulations.

Human Astrocyte (HA, NHA) Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting Human Astrocytes. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for Human Astrocytes. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Unique formulation-maximize transfection performance in Human Astrocytes.
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• 0.5 ml is able to transfect about 1000 wells of 24-well plate
• synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
• Compatible with serum
• Suitable for Reverse Transfection
• Compatible with transfection in any plate formats
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems