Cell Information:

Human Cardiac Fibroblasts (HCF, NHCF-A and -V) are derived from normal human heart tissues (atrium or ventricle).Aortic Adventitial Fibroblasts (AoAF) (isolated from the tunica external of the ascending or descending aorta) areCardiac Related fibroblasts . Human Cardiac fibroblasts or Cardiac Related Fibroblasts have been used to study the effects of TNF-alpha and angiotensin (Ang) II levels on fibroblast function. Expression of MyoD can induce myogenesis of cardiac fibroblasts. Cardiac fibroblasts involved in aortic regurgitation synthesize more Fibronectin. Estradiol inhibits cardiac fibroblast growth through conversion to methoxyestradiols, which may protect against remodeling involved in heart disease. TGF-beta stimulates differentiation of fibroblasts to myofibroblasts, which increases collagen production.

Applications:

• Vascular diseases
• Neointimal formation
• Vascular remodeling
• Atherosclerosis
• Hypertension
• Myocardial repair
• Extracellular maintenance during cardiac cell / tissue growth
• Cardiac Fibrosis Paracrine signalling
• Toxicology
• Gene expression

The Transfection Reagent:

Transfection of primary cells, such as Human Cardiac Fibroblasts or Cardiac Related Fibroblasts, is a problem in the laboratory routine and further in tissue engineering and gene therapy. Most methods working effectively for cell lines in culture fail to transfect primary cells.

Human Cardiac Fibroblasts (HCF, NHCF-A, NHCF-V, AoAF) Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on Human Cardiac fibroblasts and Cardiac Related Fibroblasts. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for Human Cardiac fibroblasts and Cardiac Related Fibroblasts. No other transfection reagents can match the efficiency, convenience, and gentleness of The Reagent for transfection on Human Cardiac fibroblasts and Cardiac Related Fibroblasts. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Unique formulation-maximize transfection performance in Human Cardiac fibroblasts and Cardiac Related Fibroblasts.
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• 0.5 ml is able to transfect about 1000 wells of 24-well plate
• synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
• Compatible with serum
• Suitable for Reverse Transfection
• Developed and manufactured by EZ Biosystems