Cell Information:
Chondrocytes produce and maintain the extracellular matrix of cartilage. Human Chondrocytes are usually derived from normal human articular cartilage. Human Chondrocytes growing in serial monolayer culture lose their phenotypic markers and became de-differentiated. This de-differentiation stage can be reversed by culturing them in semi-solid gel. Human Chondrocyte has served as a useful model for studying chondrocyte differentiation and the effects of cytokines and growth factors on the suppression and maintenance of differentiated cartilage phenotype.
Applications:
- Osteoarthritis
- Cartilage failure
- Cellular matrix linkage rupture
- Chondrocyte protein synthesis inhibition
- Chondrocyte apoptosis
The Transfection Reagent:
Transfection of primary cells, such as HC, is a problem in the laboratory routine and further in tissue engineering and gene therapy. Most methods working effectively for cell lines in culture fail to transfect primary cells.
Human Chondrocyte (HC, NHAC, HC-RA, HC-OA) Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on HC. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for HC. No other transfection reagents can match the efficiency, convenience, and gentleness of Human Chondrocyte (HC, NHAC, HC-RA, HC-OA) Avalanche™ Transfection Reagent for transfection on HC. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?
Features:
- Unique formulation-maximize transfection performance in HC.
- Highest efficiency to ensure experimental success
- Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
- 0.5 ml is able to transfect about 1000 wells of 24-well plate
- Deliver single or multiple plasmids
- Suitable for Reverse Transfection
- Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
- Developed and manufactured by EZ Biosystems
