Cell Information:
Fibroblasts are found in all connective tissues, and they synthesize and secrete extracellular matrix proteins under cell culture conditions. Human Dermal Fibroblasts (HDF)are derived from the dermis of normal human neonatal foreskin or adult skin. They are a well established system for in vitro analysis of fibroblast growth, migration and collagen metabolism in wound healing. Fibroblasts grown in a biodegradable mesh have been used as a living dermal replacement.
Applications:
- ECM protein analysis
- Wound healing
- Collagen metabolism
- Cosmetics
- Skin therapy/models
The Transfection Reagent:
Transfection of primary cells, such as HDF, has always been challenging, both technically and financially. The most common systems currently used for molecular delivery to these cells include expensive electroporation instruments and relatively toxic chemical formulations.
Human Dermal Fibroblast (HDF) Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on HDF. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for HDF. No other transfection reagents can match the efficiency, convenience, and gentleness of Human Dermal Fibroblast (HDF) Avalanche™ Transfection Reagent for transfection on HDF. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?
References:
- Seong RK, Seo SW, Kim JA, Fletcher SJ, Morgan NV, Kumar M, Choi YK, Shin OS.Schlafen 14 (SLFN14) is a novel antiviral factor involved in the control of viral replication.Immunobiology. 2017 Jul 11. pii: S0171-2985(17)30115-8. doi: 10.1016/j.imbio.2017.07.002. [Epub ahead of print] PubMed PMID: 28734654.
- Kim JA, Park SK, Seo SW, Lee CH, Shin OS.STING is Involved in Antiviral Immune Response against VZV Infection via the Induction of Type I and III IFN Pathways.J Invest Dermatol. 2017 Jun 21. pii: S0022-202X(17)31660-3. doi: 10.1016/j.jid.2017.03.041. [Epub ahead of print] PubMed PMID: 28647346.
Features:
- Specifically optimized to deliver nucleic acids into HDF
- Highest efficiency to ensure experimental success
- Extremely gentle to cells
- 0.5 ml is able to transfect about 1000 wells of 24-well plate
- Deliver single or multiple plasmids
- synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
- Compatible with serum
- Compatible with transfection in any plate formats
- Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
- Economical: High efficiency means less amount of nucleic acid & reagent is needed
- Developed and manufactured by EZ Biosystems
