Cell Information:

Human Epidermal Melanocytes (HEM) are the pigment producing cells located in the epidermal layers of all mammalian skin. The function of the melanocyte is to produce melanin, the pigment that gives skin its color. Melanin protects skin from UV damage.

Melanocytes in cosmetic studies are used to test:

• Skin lightening compounds for moles-freckles-nevi-age spots
• Skin darkening products - tan enhancing products
• UV protection compounds - sunscreen

Melanocyte in medical research often centers on melanoma as well as various dermatological diseases or related disorders like:

• Albinism and vitiligo
• Hormonal treatments
• Thyroid conditions
• Psoriasis
• Burns
• Scars

The Transfection Reagent:

Transfection of primary cells, such as HEM, is a problem in the laboratory routine and further in tissue engineering and gene therapy. Most methods working effectively for cell lines in culture fail to transfect primary cells.

Human Epidermal Melanocyte (HEM, NHEM) Avalanche™ TRansfection Reagent is a new, proprietary solution specifically designed for transfection on HEM. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for HEM. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Specifically optimized to deliver plasmid DNA into HEM
• Highest efficiency to ensure experimental success
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• Much less reagent needed for each transfection: 0.5 ml is able to transfect about 1000 wells of 24-well plate
• Simplest and straightforward protocols
• Compatible with transfection in any plate formats
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems